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How to Prepare a Tris Buffer Solution Tris-hydroxymethyl aminomethane (TRIS) is widely used for the preparation of buffers in biochemical research. Shown here is the structure of TRIS in its protonated form: ^ NH3 HOCH2- C-CH2OH | CH2OH Its acid dissociation constant, Kα, is 8.32 ×10^-9 M. You have available at your lab bench a 0.1 M solution of TRIS in its protonated form, 0.1 M solutions of HCl and NaOH, and ample distilled water. Describe the preparation of a 1-L soluti
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