In this work, we establish the use of nucleic acid hybridization to program the activation of catalysis in enzymes. We do this via “thiol switching”, using thiolated oligonucleotides: a protein is ...
Cell-free protein expression (CFE) systems have emerged as a critical platform for synthetic biology research. The vectors for protein expression in CFE systems mainly rely on double-stranded DNA and ...
The Polymerase Chain Reaction (PCR) is a technique for the amplification of DNA in vitro (this describes experiments with cells outside their normal environment). PCR amplifies DNA using complementary ...